Carbon isotopes and water use efficiency in C4 plants

Abstract

Drought is a major agricultural problem worldwide. Therefore, selection for increased water use efficiency (WUE) in food and biofuel crop species will be an important trait in plant breeding programs. The leaf carbon isotopic composition (δ13Cleaf) has been suggested to serve as a rapid and effective high throughput phenotyping method for WUE in both C3 and C4 species. This is because WUE, leaf carbon discrimination (Δ13Cleaf), and δ13Cleaf are correlated through their relationships with intercellular to ambient CO2 partial pressures (Ci/Ca). However, in C4 plants, changing environmental conditions may influence photosynthetic efficiency (bundle-sheath leakiness) and post-photosynthetic fractionation that will potentially alter the relationship between δ13Cleaf and Ci/Ca. Here we discuss how these factors influence the relationship between δ13Cleaf and WUE, and the potential of using δ13Cleaf as a meaningful proxy for WUE.