Development of Biogenic Silver Nano Particle from Pelargonium Graveolens Leaf Extract and their Antibacterial Activity

Abstract

Our research focused on the production, characterization and application of\rsilver nanoparticles (AgNPs), which can be utilized in biomedical research and\renvironmental cleaning applications. We used an environmentally friendly extracellular\rbiosynthetic technique for the production of the AgNPs. The reducing agents used to\rproduce the nanoparticles were from aqueous extracts made from the leaves of various\rplants. Synthesis of colloidal AgNPs was monitored by UV-Visible spectroscopy.\rThe UV-Visible spectrum showed a peak between 417 and 425 nm corresponding to the\rPlasmon absorbance of the AgNPs. The characterization of the AgNPs such as their size\rand shape was performed by Atom Force Microscopy (AFM), and Transmission Electron\rMicroscopy (TEM) techniques which indicated a size range of 3 to 15 nm. The anti-bacterial\ractivity of AgNPs was investigated at concentrations between 2 and 15 ppm for\rGram-negative and Gram-positive bacteria. Staphylococcus aureus and Kocuria rhizophila,\rBacillus thuringiensis (Gram-positive organisms); Escherichia coli, Pseudomonas aeruginosa,\rand Salmonella typhimurium (Gram-negative organisms) were exposed to AgNPs using\rBioscreen C. The results indicated that AgNPs at a concentration of 2 and 4 ppm, inhibited bacterial growth. Preliminary evaluation of cytotoxicity of biosynthesized silver\rnanoparticles was accomplished using the InQ™ Cell Research System instrument with\rHEK 293 cells. This investigation demonstrated that silver nanoparticles with a concentration\rof 2 ppm and 4 ppm were not toxic for human healthy cells, but inhibit bacterial growth.