Scatchard plots for ethylene binding in apples (Malus domestica Borkh.), which were harvested weekly for 5 weeks to include the ethylene climacteric rise, showed C 50 values (concentration of ethylene needed to occupy 50% of the ethylene binding sites) of 0.10, 0.11, 0.34, 0.40, and 0.57 μl ethylene/liter -1 , respectively, for each of the 5 weeks. Higher ethylene concentrations were required to saturate the binding sites during the climacteric rise than at other times. Diffusion of 14 C-ethylene from the binding sites was curvilinear and did not show any indication of multiple binding sites. Ethylene was not metabolized by apple tissue.
CITATION STYLE
Blankenship, S. M., & Sisler, E. C. (2019). Ethylene Binding Site Affinity in Ripening Apples. Journal of the American Society for Horticultural Science, 118(5), 609–612. https://doi.org/10.21273/jashs.118.5.609
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