Determination of lactose in milk by gas liquid chromatography

Abstract

A method was adapted to determine lactose in milk by gas liquid chromatography (GLC) of trimethylsilyl ethers. Quantitation was accomplished by comparison to an internal standard, sorbitol. The standard deviation was 0.132% lactose, and the procedure was compared to a recognized method which employed chloramine T. The GLC method worked well with either milk or skimmilk.