Follicular growth and atresia in the ovaries of hens (Gallus domesticus) with diminished egg production rates

Abstract

Diets containing 3.5, 1.0 and 0.1% calcium were fed from the age of 42 weeks to individually caged laying hens. Ovaries were examined at 46-49 and 70 weeks of age for changes in the follicular population corresponding to the lowered egg production rates of birds given calcium-deficient diets (1.0% and 0.1%) and older birds given a normal diet (3.5%). Growth rates of follicles from 3.5 mm diameter to ovulation were not changed by the level of dietary calcium in 46-49-old birds. The number of atretic small follicles (≤8 mm diam.) increased in old and calcium-deprived birds, resulting in lower numbers of viable follicles in the intermediate stages of growth (3-8 mm diam.). There was also an increase in the number of small follicles (1-2 mm diam.) starting to grow in 70-week-old birds which may have partly compensated for the increased loss by atresia. Birds of all ages on all diets were able to produce large follicles up to ovulable size. The main feature of poor laying birds was a reduction in the ovulation rate due to the loss of large follicles (>8 mm diam.) by atresia, an event seen rarely in the birds with good laying performance. As atresia is the normal fate of most of the small follicles, the mechanisms controlling atresia in the small follicles and the large follicles appear to be independent.