Synovial membrane mesenchymal stem cell [SM-MSC] induced IGF-1 promote growth factor release in conditioned medium SM-MSC

Abstract

In the process of in vitro culture, mesenchymal stem cells [MSC] release some secretions, resulting in a variety of growth factors and cytokines in the growth medium of MSCs. These media are called conditioned media [CM] and are currently considered to be a better alternative to stem cell therapy. To increase the potential of CM-MSCs in regenerative therapy, in this study we induced the SM-MSCs with IGF-1 to promotes growth factors and cytokine release in CM-SMMSCs. After SM-MSCs were treated with IGF-1, the CM was collected and BMP-1, FGF-18, and TGF-β1 were analyzed using ELISA method. Based on the results, the most significant increases in BMP-2, FGF18, and TGF-β1 protein concentrations were found in SM-MSCs-CM cultured with 150 ug / mL of IGF-1. Therefore, 150 μg / ml of IGF-1 is a potential concentration used for induced growth factors released in CM-SMMSCs.