A novel biosensor to detect microRNAs rapidly

Abstract

δ-free F0F1-ATPase within chromatophore was constructed as a novel biosensor to detect miRNA targets. Specific miRNA probes were linked to each rotary δ subunits of F0F 1-ATPase. Detection of miRNAs was based on the proton flux change induced by light-driven rotation of δ-free F0F 1-ATPase. The hybridization reaction was indicated by changes in the fluorescent intensity of pH-sensitive CdTe quantum dots. Our results showed that the assay was attomole sensitivities (1.2 × 10-18 mol) to target miRNAs and capable of distinguishing among miRNA family members. Moreover, the method could be used to monitor real-time hybridization without any complicated fabrication before hybridization. Thus, the rotary biosensor is not only sensitive and specific to detect miRNA target but also easy to perform. The δ-free F0F1-ATPase-based rotary biosensor may be a promising tool for the basic research and clinical application of miRNAs. Copyright © 2009 Jie-Ying Liao et al.