Abstract
The Endoplasmic Reticulum (ER)-Golgi apparatus of plants is the site of synthesis of non-cellulosic polysaccharides that then traffic to the cell wall. A two-step protocol of flotation centrifugation followed by free-flow electrophoresis (FFE) resolved ER and Golgi proteins into three profiles: an ER-rich fraction, two Golgi-rich fractions, and an intermediate fraction enriched in cellulose synthases. Nearly three dozen Rab-like proteins of eight different subgroups were distributed differentially in ER- vs. Golgi-rich fractions, whereas seven 14-3-3 proteins co-fractionated with cellulose synthases in the intermediate fraction. FFE offers a powerful means to classify resident and transient proteins in cell-free assays of cellular location.
Author supplied keywords
Register to see more suggestions
Mendeley helps you to discover research relevant for your work.
Cite
CITATION STYLE
Okekeogbu, I. O., Aryal, U. K., Fernández-Niño, S. M. G., Penning, B. W., Heazlewood, J. L., McCann, M. C., & Carpita, N. C. (2019). Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus. Plant Signaling and Behavior, 14(12). https://doi.org/10.1080/15592324.2019.1672513
Readers' Seniority
Readers' Discipline
