In this article, we focus on a refinement of the traditional voltage-clamp methods that are used to measure current from whole cells, or relatively large areas of membrane, called the patch-clamp technique. Although this technique has extended the application of voltage-clamp methods to the recording of ionic currents flowing through single channels, in its whole-cell configuration it has become the most widely used method for recording ionic currents. We give particular attention to the study of voltage-gated (CaV) Ca2+ channels using the patch-clamp technique and discuss some aspects of the molecular physiology of these proteins. © 2014 Cold Spring Harbor Laboratory Press.
CITATION STYLE
Gandini, M. A., Sandoval, A., & Felix, R. (2014). Patch-clamp recording of voltage-sensitive Ca2+ channels. Cold Spring Harbor Protocols, 2014(4), 329–335. https://doi.org/10.1101/pdb.top066092
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