EFFECT OF COLLAGEN CONCENTRATION ON MORPHOLOGY OF PVA/CHITOSAN FIBERS MADE BY ELECTROSPINNING METHOD

Abstract

The purpose of this study was to determine the impact of the addition of collagen concentration on morphology of PVA/chitosan nanofiber. The research was conducted by making 10% PVA solution, 2% chitosan solution, and collagen solutions with various concentrations of 2%, 4%, and 8%. The PVA/chitosan/collagen nanofibers were made with ratios of 8:2:2, 8:2:4, and 8:2:8 and were named P8/Ch2/C2, P8/Ch2/C4, and P8/Ch2/C8. As a control was PVA/chitosan with a ratio of 8:2, which was named P8/Ch2/C0. The nanofiber was made by electrospinning method with the following parameters: a voltage of 10 kV, a distance from the spinneret tip to the collector of 10 cm, and a flow rate of 1.00 µl/h. Nanofiber characterization was carried out using FTIR and SEM. SEM images were analyzed using ImageJ and Origin to measure the diameter and length of the fibers. It obtained that, the best ratio of PVA/chitosan/collagen to make a good nanofiber is 8:2:2, with the characteristics are the least number of beads and solution droplets, the most continuous fiber, and the average diameter is 145.55±8.64 nm. This result showed that, the addition of collagen can improve the morphology of PVA/chitosan nanofibers, where the characteristics of fibers made depending on the concentration of collagen added.