Effects of statins on adhesion molecule expression in endothelial cells

Abstract

Background: Inhibitors of HMG-CoA reductase are widely used to prevent atherosclerosis progression. The expression of adhesion molecules on activated endothelial cells (EC) is an important step in the initiation and progression of atherosclerosis. Objectives: We investigated whether adhesion molecule expression on activated EC is influenced by simvas-tatin, fluvastatin and pravastatin and, if so, by which mechanisms. Methods: Human EC from umbilical veins or saphenous veins were pretreated overnight with statins with or without mevalonate, and also for simvastatin or fluvastatin with the isoprenoid intermediates, farnesyl pyrophosphate (FPP), or geranylgeranyl pyrophosphate (GGPP). After 4-6 h activation with tumor necrosis factor (TNF)-a or lipopolysaccharide (LPS), surface adhesion molecule expression was evaluated by ELISA and by flow cytometry. The same experiments were performed with selective inhibitors of geranylgeranyltransfer-ase (GGTI-286) and farnesyltransferase (FTI-277). Results'. Pretreatment with simvastatin, fluvastatin or pravastatin potentiated the TNF-α and LPS-induced expression of E-se-lectin and VCAM-1, and mevalonate reversed the potentiating effect of these statins. GGPP also reversed the potentiating effect of simvastatin or fluvastatin on adhesion molecule expression, while FPP only partially reversed this effect. Furthermore, GGTI-286, but not FTI-277, mimicked the effect of simvastatin by increasing the TNF-α-mediated overexpression of E-selectin. Conclusions: Statins increase E-selectin-and VCAM-1-induced expression on vascular endothelial cells stimulated with TNF-α or LPS. The inhibition of geranylger-anylated proteins could contribute to this effect. © 2003 International Society on Thrombosis and Haemostasis.