The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specifi c antibodies in blood sera was performed by the virus neutralization test (VNT), and identifi cation of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log2. High seroprevalence was confi rmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confi rmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specifi c antibodies was confi rmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
CITATION STYLE
Sava, L., Diana, L., Vladimir, P., Miroslav, V., Gospava, L., Enisa, P. S., & Tama’, P. (2015). Detection of equine arteritis virus in the semen of stallions in the republic of serbia. Acta Veterinaria, 65(4), 557–567. https://doi.org/10.1515/acve-2015-0047
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