Separation of dansyl amino acid enantiomers by ligand-exchange capillary electrochromatography using an L-lysinamide-modified monolithic silica column

Abstract

An L-lysinamide-modified monolithic column has been prepared by a sol-gel process and chemical modifications, and used for the enantioseparation of dansyl amino acids by both modes of capillary electrochromatography (CEC) and micro-high performance liquid chromatography (μ-HPLC). The electroosmotic flow (EOF), separation conditions and enantioselectivity have been investigated. The chiral selector of L-lysinamide loaded with Cu(II) ion generates a strong EOF, probably due to the nature of lysinamide containing two amino groups in a molecule. Besides, the separation behaviors in CEC and μ-HPLC have been compared.