Karakterisasi Enzim Selulase PMP 0126Y dari Limbah Pengolahan Agar

Abstract

From previous screening study, PMP 0126 has been identified as one of potentially isolate from medium scale agar processing industries in Pamengpeuk, Garut. However, PMP 0126 was not pure yet, containing 2 isolates PMP 0126 Y and PMP 0126W. PMP 0126 Y produced higher celullase compared to the PMP 0126W. Aims of this study was to produce and characterize PMP 0126Y cellulase and identify the isolate. Result showed that the cellulase was optimally produced on the third days of cultivation using 1% CMC medium. Crude enzyme worked optimally at temperature of 30 °C and pH 5, and the activity could be improved by addition of 5 mM CaCl2 and ZnCl2 . Purification using anion exchanger could improve the activity 15 times with yield of 20%. PMP 0126Y produced 3 cellulases with estimated molecular weight of 39, 30 dan 14 kDa. The crude enzyme could degrade the agar processing waste as good as CMC, indicating that this enzyme could be used as saccharification agent for bioethanol processing from agar processing waste. Identification study