The class A scavenger receptor binds to proteoglycans and mediates adhesion of macrophages to the extracellular matrix

Abstract

The class A scavenger receptor (SR-A) binds modified lipoproteins and has been implicated in cholesterol este r deposition in macrophages. The SR-A also contributes to cellular adhesion. Using SR-A+/+ and SR-A-/- murine macrophages, we found SR-A expression important for both divalent cation-dependent and -independent adhesion of macrophages to the human smooth muscle cell extracellular matrix. The SR-A mediated 65 and 85% of macrophage adhesion to the extracellular matrix in the presence and absence of serum, respectively. When EDTA was added to chelate divalent cations, the SR-A mediated 90 and 95% of the macrophage adhesion without and with serum, respectively. SR-A-mediated adhesion to the extracellular matrix was prevented by fucoidin, an SR-A antagonist. Biglycan and decorin, proteoglycans of the extracellular matrix, were identified as SR-A ligands. Compared with control cells, Chinese hamster ovary cells expressing the SR-A showed 5- and 6-fold greater cell association (binding and internalization) of 125I-decorin and -biglycan, respectively. In competition studies, unlabeled proteoglycan or fucoidin competed for binding of 125I-labeled decorin and -biglycan, and biglycan and decorin competed for the SR-A-mediated cell association and degradation of 125I-labeled acetylated LDL, a well characterized ligand for the SR-A. These results suggest that the SR-A could contribute to the adhesion of macrophages to the extracellular matrix of atherosclerotic plaques.