SCREENING OF Andrographis Paniculata EXTRACT FOR ANTIOXIDANT AND GENOTOXIC ACTIVITIES

Abstract

Andrographis paniculata is an important medicinal plant which has been used to treat various ailments. The present study was undertaken to analyze the phytochemical compounds and evaluated the antioxidant and genotoxic potential of Andrographis paniculata leaves and whole plant extracts.   Phytochemical compounds analyzed by qualitative and quantitative analysis of methanol extracts of the leaves and whole plant extracts which showed the presence of Alkaloids, Carbohydrate, Resins, Saponins, Flavonoid, Steroids, Glycosides and Tannin. Quantitative analysis were also conducted to determine the amount of  Alkaloids, Flavonoids, Saponins and Tannin by  HPTLC Finger printing methods  in  A. paniculata ( A.P.) leaves and whole plant .The Antioxidant activity of A. paniculata Methanolic extract was evaluated by   Fenton  methods which showed the dose dependent Inhibition of TBARS formation. The Genotoxicity was evaluated by Micronucleus Assay. The dose dependent prevention of bone marrow micronucleus formation by Andrographis paniculata leaves and whole plant extracts was observed. Therefore, Andrographis paniculata leaves and whole plant extract caused antioxidant and genotoxic potential.