Identification of Bacteria from the Anaplasmataceae Family in Dog Shelter of the Municipality of Caldas, Antioquia

Abstract

Objetive. It was to detect the circulation of microorganisms of the family Anaplasmataceae in a canine albergo of the municipality of Caldas. Materials and methods. In view of the above, a descriptive study was conducted in 46 dogs (Canis lupus familiaris) to evaluate the presence of Ehrlichia spp. and Anaplasma spp. by molecular techniques or by immunodiagnostic, in a dog shelter of the municipality of Caldas. The DNA extraction was done in whole blood samples with EDTA. Primers were used against dsb and groEL genes to detect Ehrlichia and Anaplasma sequences respectively and phylogenetic analyzes of the sequences obtained were also carried out. On the other hand, SNAP 4DX® was used for serodiagnosis. Results. In the molecular testing, five positive samples were obtained for the Ehrlichia test and three for the Anaplasma test. In the serological tests, three individuals were positive for Ehrlichia spp., three for Anaplasma spp. and for the other trials measured by the SNAP (Dirofilaria immitis y Borrelia burgdorfe) the result was negative. Conclusions. It demonstrated the circulation of Ehrlichia canis and Anaplasma platys in a dog shelter of the municipality of Caldas, Antioquia.