TGF-Β1 gene-modified, immature dendritic cells delay the development of inflammatory bowel disease by inducing CD4+ Foxp3+ regulatory T cells

Abstract

Inflammatory bowel disease (IBD) is caused by an uncontrolled immune response in the intestinal lumen, leading to inflammation in genetically predisposed individuals. Immunotherapy may be a promising approach to the treatment of IBD. Here, we show that transforming growth factor-Β1 (TGF-Β1) gene-modified immature dendritic cells (imDCs) could enhance the inhibitory function of imDCs and delay the progress of IBD induced by dextran sodium sulfate in mice. The results of fluorescence-activated cell sorter (FACS) demonstrated that this protective effect is mediated partially by inducing CD4 Foxp3 regulatory T cells (Tregs) in mesentery lymph nodes to control inflammation. In vitro experiments also supported this hypothesis. In conclusion, we provide evidence that TGF-Β1-modified bone marrow-derived imDCs may have a therapeutic effect to IBD. © 2010 CSI and USTC. All rights reserved.