Specific Vβ T Cell Subsets Are Associated with Cat and Birch Pollen Allergy in Humans

Abstract

Cognate interaction between TCRs and MHC class II molecules plays an important role in initiating the allergen-specific immune response. Therefore, we analyzed the TCR distribution of human PBLs of 56 atopic and nonatopic (NA) individuals, including 4 monozygotic twin pairs, from two extended and four nuclear families. The expression of 23 Vβ and 3 Vα elements was analyzed. The blood samples of symptomatic birch pollen-sensitized individuals that were taken ≤6 wk after the birch pollen season (n = 8) showed a significantly higher frequency of Vβ16.1+ and Vβ20.1+ T cells compared with the blood samples of birch pollen-sensitized individuals that were obtained out of allergen season (n = 10) or from NA individuals (p < 0.0005 and p < 0.0001, respectively). Allergen-specific lymphocyte proliferation was detected in the allergic individuals, and the distribution of Vβ16.1+ and Vβ20.1+ T cells returned to normal levels after the pollen season. The frequency of these Vβ-expressing T cells correlated with the levels of allergen-specific IgE Abs. In addition, cat-sensitized individuals (n = 8) showed a significantly higher frequency of Vβ17.1-expressing T cells than did NA individuals (p < 0.005). Our results indicate restricted TCR-Vβ gene usage in cat and birch pollen allergies; we suggest that both genetic and environmental factors contribute to TCR-Vβ gene expression and to the development of a specific T cell response.