Ca2+-induced Ca2+ release in chromaffin cells seen from inside the ER with targeted aequorin

Abstract

The presence and physiological role of Ca2+induced Ca2+ release (CICR) in nonmuscle excitable cells has been investigated only indirectly through measurements of cytosolic [Ca2+] ([Ca2+](c)). Using targeted aequorin, we have directly monitored [Ca2+] changes inside the ER ([Ca2+](ER)) in bovine adrenal chromaffin cells. Ca2+ entry induced by cell depolarization triggered a transient Ca2+ release from the ER that was highly dependent on [Ca2+](ER) and sensitized by low concentrations of caffeine. Caffeine-induced Ca2+ release was quantal in nature due to modulation by [Ca2+](ER). Whereas caffeine released essentially all the Ca2+ from the ER, inositol 1,4,5-trisphosphate (InsP3) producing agonists released only 60-80%. Both InsP3 and caffeine emptied completely the ER in digitonin-permeabilized cells whereas cyclic ADP-ribose had no effect. Ryanodine induced permanent emptying of the Ca2+ stores in a use-dependent manner after activation by caffeine. Fast confocal [Ca2+](c) measurements showed that the wave of [Ca2+](c) induced by 100-ms depolarizing pulses in voltage-clamped cells was delayed and reduced in intensity in ryanodine- treated cells. Our results indicate that the ER of chromaffin cells behaves mostly as a single homogeneous thapsigargin-sensitive Ca2+ pool that can release Ca2+ both via InsP3 receptors or CICR.