Inhibition effectivity of Halimeda macroloba seaweed extract against fish indigenous bacteria for safety fisheries product

Abstract

Concerns about the potential for residual antibiotics can cause adverse health effects for consumers. The use of inappropriate antibiotics is feared to lead to antimicrobial resistance by pathogens. New sources of antibacterial can be obtained from bioactive compounds from seaweed, one of which is a H. macroloba type. The research objective was to determine the effectiveness of H. macroloba seaweed extract inhibition against indigenous bacteria in fish. H. macroloba samples taken from the waters of Pane Island. The extraction process was carried out by maceration method with methanol p.a solvent. The secondary metabolites were obtained with phytochemical test. Inhibition test with the Kirby-Bauer agar diffusion method through experimental by adding DMSO. H. macroloba have secondary metabolite compounds, there are alkaloids and flavonoids which a function as an inhibitory force for bacteria. Different treatment of H. macroloba extract resulted in a significant effect on Vibrio parahemolitycus with an inhibitory value of 10.73 mm. Meanwhile, different treatment on Aeromonas hydrophyla and Aeromonas salmonicida was stated to have no significant effect in increasing the inhibition zone diameter. However, it was directly proportional to the increasing effectiveness of the inhibition zone diameter, the most effective treatment was at a concentration of 100%.