CURCUMINOID NANOEMULSION FROM Curcuma xanthorrhiza EXTRACT AND ITS ACTIVITY AS ANTIOXIDANT, ANTIBACTERIAL AND ANTIFUNGAL

Abstract

Curcuminoids are the main compounds in the rhizome extract of C. xanthorrhiza which are beneficial for health. The aim of the research was to study the formulation of curcuminoid nanoemulsions and their biological effects as antioxidant, antibacterial, and antifungal agents. The formulation of the curcuminoid nanoemulsion was carried out using the spontaneous emulsion method, by mixing the curcuminoid extract with oil and tween 80 and adding phosphate buffer pH 7.0. Curcuminoid nanoemulsion products were characterized and tested for their potential biological activity. Antioxidant test using reagent 2,2-diphenyl-1-picrylhydrazyl. Antibacterial test using diffusion method against Escherichia coli and Staphylococcus epidermidis bacteria. Antifungal test using Candida albicans. The curcuminoid nanoemulsion product showed a particle size of 19.5 to 32.1 nm, a polydispersity index < 0.3, and a zeta potential of-4.5 to-9.2 mV. Curcuminoid nanoemulsions have high antioxidant and antifungal activity, while antibacterial activity showed moderate activity. The conclusion is that curcuminoid nanoemulsions from C. xanthorrhiza extract can be used effectively as antioxidants and antimicrobial for commercial applications.