Effects of dayak onion bulbs (Eleutherine bulbosa (Mill.) urb) on bone development of the hipoestrogen model rat

Abstract

Background: Eleutherinol is a naphtoquinone derivative that has strong affinity to bind with estrogen alpha receptors (ERα). This compound can be found in Dayak Onion Bulbs (Eleutherine bulbosa Mill.). Objective: The purpose of this study is to analyze the effects of Dayak Onion Bulbs extract on the growth of the bone of hypoestrogen model rat. Methods: A total of 36 female white rats of Sprague-Dawley were divided into 6 groups. All 6 groups receive CMC Na 0,5% (sham group), CMC Na 0,5% (negative group), tamoxifen (positive group), three level of dayak onion bulbs extract doses namely dose 8 mg/ 200 g, 12 mg/ 200 g, and 18 mg/ 200 g. All groups, except the sham, is ovariectomized to obtain the conditions of hypoestrogen. After ovariectomy, all rats were evaluated for the success of ovariectomy on day 35, followed by the administration of the extracts by oral administration for 21 days. After 21 days administration, the level of bone calcium, bone weight, and bone length were measured by Atomic Absorption Spectrophotometry. Results: Dayak onion bulbs extract at dose 3 (18 mg/200 g BW) could increase significantly bone calcium level, bone weight, and bone legnth of hypoestrogen rat. Conclusion: The results showed that Dayak onion bulbs extract could be developed to be a drug for menopause woman in the future.