Antioxidant activities of aqueous and 70% ethanol extracts of akar kuning (Arcangelisia flava (L.) Merr) stem using the DPPH method

Abstract

Background: Numerous herbs can be used as natural exogenous antioxidants and clinically proven effective as antioxidants. Objective: The primary objective of this study is to assess the antioxidant activity of the stems of akar kuning (Arcangelisia flava (L.) Merr). Method: This plant was extracted with aqueous solution using the decoction method and 70 % ethanol using the maceration method. Antioxidant activity was evaluated using the TLC-Bioautography sprayed with DPPH reagent for the qualitative method and microplate assay with DPPH for the quantitative method. This research also identified the total phenol from each extract which was measured using the Folin-Ciocalteu method. Result: Based on phytochemical screening, each extract contained flavonoids, polyphenols, anthraquinones, terpenoids, and saponins. TLC-bioautography showed flavonoids, polyphenols, and anthraquinones were responsible for antioxidant activity. Quantification of antioxidant activity results of aqueous and 70% ethanol extracts of akar kuning (Arcangelisia flava (L.) Merr) showed IC50 value at 78.8 ± 1.2 µg/mL and 142.8± 2.9 µg/mL respectively. The content of total phenolic compounds was 80.544 ± 2.6 mg GAE/ g and 52.522 ± 1.9 mg GAE/g. Conclusion: As observed in the study, Arcangelisia flava (L.) Merr extracts from both extraction methods possess an antioxidant.