Growth and development of vanda (Vanda sanderiana) explants in vitro on the effect of extracts of organic matter

Abstract

This study aims to determine the appropriate extract of organic matter to be substituted on MS media, to obtain Vanda orchid Vanda sanderiana plantlets ready for acclimatization. The study consisted of 2 experiments, the first experiment, factorial Completely Randomized Design (CRD), the first factor was tomato extract, consisting of 3 levels (tomato extract 50 ml/l; tomato extract 100 ml/l, and tomato extract 150 ml/l) and the second factor consists of 3 levels of bean sprouts extract (100 ml/l bean sprouts extract, 150 ml/l bean sprouts extract, 200 ml/l bean sprouts extract). The second experiment compared the best or most efficient composition of the first experiment with BAP media (3 ppm), using the T-test. In the first experiment the growth variables (plant height and number of leaves) of all treatments were not significantly different, but the highest number of shoots was obtained at 50 ml/l tomato extract and 100 ml/l bean sprouts extract (6 shoots), the other 1-2 shoots. This treatment in the second experiment was proven to show a better plant height, leaf number and number of shoots than plant growth in BAP media (3 ppm).