Agonistic behaviour and energy metabolism of bold and shy swimming crabs Portunus trituberculatus

Abstract

The metabolism and behaviour of crustaceans are highly flexible, and the inter-individual variation in these traits is evolutionarily and ecologically significant. We analysed the relationships among personality traits (boldness, activity and hesitancy), agonistic behaviour and energy status (glycogen, glucose and lactate) in the swimming crab Portunus trituberculatus. The main results were as follows. (1) Boldness was significantly correlated with activity and hesitancy. Bold crabs were more likely to initiate and win a fight. In bold individuals, the frequencies of ‘move to’, ‘cheliped display’, ‘grasp’ and ‘contact’ were significantly higher than those of shy individuals, whereas the frequency of ‘move away’ was significantly lower than that of shy individuals. (2) Before fighting, the glucose concentrations in the haemolymph of bold individuals were significantly lower than those of shy individuals, whereas the concentrations of lactate showed the opposite trend. There were no significant differences in glycogen and lactate concentrations in the claw muscle between bold and shy individuals. (3) After fighting, the glucose and lactate concentrations in the haemolymph of both bold and shy individuals were significantly higher than those before fighting. The glucose concentrations in the haemolymph were significantly higher in bold individuals than shy individuals. In addition, bold individuals showed a larger increase in glucose in the haemolymph but a smaller increase in lactate compared with shy individuals. (4) After fighting, the glycogen concentrations in the claw muscle were significantly lower than those before fighting; however, there were no significant differences in the concentrations of lactate in the claw muscle. These results indicated that the agonistic behaviour of the swimming crab is related to its behavioural type. Energy reserves may be one of the factors affecting the personality traits and agonistic behaviour in crabs. These results should lay a foundation for in-depth understanding of the relationships among crustacean personality, agonistic behaviour and metabolic physiology.