CLAE: A High-Fidelity Nanopore Sequencing Strategy for Read-Level Viral Variant Detection and Environmental RNA Virus Discovery

Abstract

High-fidelity (HF) long-read sequencing enables accurate profiling of microorganisms and pathogens at single-molecule resolution. However, current Oxford Nanopore Technologies (ONT)—a revolutionary platform offering real-time, portable sequencing at relatively low instrumental cost—suffer from severe read-length bias, limited accuracy (often