Molecular Genetic Variations and Phylogenetic Relationship Using Random Amplified Polymorphic DNA of three species of Catfishes (Family: Schilbidae) in Upper Egypt

Abstract

The RAPD-PCR analysis was carried out on three species of fresh water fishes of family Schilbidae (Schilbe mystus, Schilbe uranoscopus and Siluranodon auritus) by using twenty primers. All twenty primers amplified successfully on the genomic DNA extracted from all studied fish species. The number of bands was variable in each species. Schilbe mystus produced number of bands 174, and Schilbe uranoscopus 193, while in Siluranodon auritus 210 bands. A total of 295 DNA bands were generated by all primers in all specimen, out of these DNA bands 97 (32.88%) were conserved among all specimens, while 198 bands were polymorphic with percentage 67.12% of all the twenty tested primers produced polymorphism in all specimens table 22. These results are discussed in relation to implications of RAPD assays in the evaluation of genetic diversity.