Expression of nerve growth factor and its receptors trkA and p75 and inhibin α-subunit in the ovarian interstitial cells of lactating golden hamsters

Abstract

Characteristic daily increases in the plasma levels of luteinizing hormone (LH) are present every afternoon during lactation in golden hamsters. The objective of this study was to investigate the effect of the diurnal rhythm of increases in LH on expression of nerve growth factor (NGF), its receptors trkA and p75 and inhibin α-subunit in the ovarian interstitial cells of lactating golden hamsters. Both lactating and non-lactating groups of postpartum golden hamsters were used in this study. The expression of NGF, its receptors trkA and p75 and inhibin α-subunit were determined by immunohistochemistry. Positive staining of NGF, trkA and p75 was found in the interstitial cells of the lactating group, and no immunoreactivity for NGF, trkA or p75 was observed in the ovarian interstitial cells of the nonlactating group. In addition, immunostaining of inhibin α-subunit was also observed in the interstitial cells of the lactating group but not in those of the non-lactating group. Immunostaining of the inhibin/activin βA- and βB-subunits was observed in the granulosa cells of antral follicles, but not in the interstitial cells of the lactating and non-lactating animals. These results suggest that the diurnal rhythm increases in LH can induce expression of NGF, trkA, p75 and inhibin α-subunit in the ovarian interstitial cells of lactating golden hamsters and that NGF, its receptors trkA and p75 and inhibin α-subunit may have the capacity for autocrine or paracrine modulation of interstitial cell differentiation in golden hamsters.