A Novel Validated Simultaneous Equations Method for Simultaneous Estimation of Omeprazole and Ondansetron in Bulk and Pharmaceutical Preparation

Abstract

A simple, rapid, fast, accurate, economical and precise UV spectrophotometric method based on simultaneous equations was developed and validated for simultaneous estimation of Omeprazole and Ondansetron in tablet dosage form. Methanol was selected as a solvent for the proposed method. The method involved simultaneous equations using two wavelengths, with one being the λmax of Omeprazole (205 nm) and the other being the λmax of Ondansetron (246 nm). Beer’s law was obeyed in the concentration range of 2 – 12 µg/mL for both drugs with correlation coefficients of more than 0.99. The results of analysis have been validated statistically and by recovery studies as per ICH guidelines. The limit of detection and limit of quantification were found to be 0.29 µg/mL and 0.90 µg/mL for Omeprazole, respectively and 0.07 µg/mL and 0.21 µg/mL, respectively for Ondansetron. The mean percent recovery of triplicate samples at each level for both drugs was 99.48 - 100.3% for Omeprazole and 99.49 - 99.66% for Ondansetron. The assay results are in good agreement with the label claim; hence it could be used in routine analysis of laboratory samples and marketed formulations containing these two drugs.