Studies of phospholipase C (heat-labile hemolysin) in Pseudomonas aeruginosa

Abstract

The chromogenic substate p-nitrophenylphosphorylcholine was used to measure phospholipase C (heat-labile hemolysin) production by clinical strains of Pseudomonas aeruginosa. Analysis of strains from various types of infection showed that phospholipase C production by urinary tract isolates was significantly greater than that by lung, blood, or other isolates (e.g., wounds etc.). The above method was also found to be effective for the isolation of several classes of phospholipase C mutants.