Improvement in Production and Regeneration of Somatic Embryos from Mature Seed of Melon (Cucumis melo L.) on Solid Media.

Abstract

Induction of somatic embryos from mature seed of melon (Cucumis melo. L.) was conducted on media solidified with gelrite. The most reproducible result was obtained with rhombic explant consisted of radicle, hypocotyl, and a proximal part of cytoledon. Somatic embryos were produced around the cut surface at the border of hypocotyl and cotyledon. Soaking sterilized seed in water for two hours or more promoted embryogenesis. Numbers of somatic embryos were increased by the supplementation of 2 mg/l NAA to modified MS media together with 1 approximately 4 mg/l 2,4-D and 0.1 mg/l BA. Percentages of germination (rooting and shoot formation) were different among various shapes of somatic embryos. 'Thin cone' shaped embryo germinated in high percentage. Regenerated plants grew normally and set fruits.