Palladium Nanoparticles/Graphitic Carbon Nitride Nanosheets-Carbon Nanotubes as a Catalytic Amplification Platform for the Selective Determination of 17α-ethinylestradiol in Feedstuffs

Abstract

A new kind of nanocomposite, graphitic carbon nitride (g-C3N4)-carbon nanotubes (CNTs), has been synthesized via solid grinding, and followed by thermal polymerization process of melamine and CNTs. Pd nanoparticles were loaded on the as-prepared nanocomposite by the self-assembly method. The Pd/g-C3N4-CNTs nanocomposite exhibited excellent electrocatalytic activity toward the oxidation of 17α-ethinylestradiol (EE2), and compared with other detection methods of EE2, such as HPLC, this detection platform does not need the samples for further purification processing. And this detection platform was compared with HPLC, there is no significant difference between two methods, and the accuracy and precision of the determination of EE2 in feedstuff sample by differential pulse voltammetry (DPV) to a satisfactory level. Thus, the Pd/g-C3N4-CNTs nanocomposite can be used as a signal amplification platform for the detection of EE2 in feedstuffs samples. Under the optimum condition, the current response increased linearly with EE2 concentration from 2.0 × 10−6 ~ 1.5 × 10−4 M with a detection limit of 5.0 × 10−7 M (S/N = 3) by DPV. The Pd/g-C3N4-CNTs showed good reproducibility and excellent anti-interference ability that the relative standard deviation was 3.3% (n = 5). This strategy may find widespread and promising applications in other sensing systems involving EE2.