Effect of growth regulator in micropropagation of Catharanthus roses in vitro

Abstract

The experiment was carried out in the plant tissue culture laboratory of the Horticulture and landscape department - College of Agriculture University of Kerbela during 2017-2018 to investigate the effect of growth regulators in the micropropagation of the Catharanthus roseus iv vitro. The culture of the branches of the Catharanthus roseus plants were obtained by cultivating the shoot tips of the branches of 1 cm length obtained from the developing plants growing in the field. After sterilization and culturing on MS medium supplied with different concentrations (0, 1, 2 and 3) of BA and Kin in separate experiment with steady concentration of 0.5 mg / l of IAA. The results showed a difference in the rate of studied characters according to the type and concentration of cytokines used. The BA in the concentration of 3 mg / L results were significantly higher in the studied traits, including the number and length of the vegetative branches and the soft and dry weight of the vegetative system, it achieved a rate of (21.66 branch / plantlet, 7.00 cm, 1.40 g, 0.71 g) respectively, while the Kin at the same concentration achieved the highest rate of studied traits of (15.66 branch / plantlet, 5.66 cm, 0.93 g, 0.40 g) respectively. The results of the study also showed a difference in the rate of studied traits, which included the number and length of roots and the soft and dry weight of the root total, depending on the type and concentration of auxien used. The IBA result was superior at 1.5 mg / L and with a constant concentration of 0.5 mg / l BA in achieving the highest rate of (36.33 root / shoot, 6.43 cm, 0.35 g, 0.23 g) respectively, while the NAA at the same concentration achieved the highest rate of studied traits (22.00 root / plant fraction, 2.53 cm, 0.21 g, 0.12 g), respectively, and the percentage of success of acclimation plants was 90%.