Optimization of enzyme-assisted extraction of antitumor polysaccharides from Hericium erinaceus mycelia

Abstract

In this study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from Hericium erinaceus mycelia (HEP) using the Box-Behnken design based on the results of single factor experiments. As a result, the optimal protocol was as follows: an extraction time of 79 min, an extraction temperature of 50°C, a pH of 5.7 and a ratio of water to raw materials of 33.4 mL/g. These optimal extraction conditions achieved the highest yield (13.9 ± 0.3%) of HEP. The purified fraction (HEP-2) was composed of glucose, galactose, mannose, fucose, arabinose, xylose and rhammnose. HEP-2 manifested an obvious inhibitory effect on the growth of HeLa cells. The overall findings implied that enzyme-assisted procedure is a superior method and that HEP-2 might be useful for developing natural and safe antitumor drugs.