Specificity, Inhibitory Studies, and Oligosaccharide Formation by β-Galactosidase from Psychrotrophic Bacillus subtilis KL88

Abstract

β-Galactosidase from psychrotrophic Bacillus subtilis KL88 wasspecific to the β-D-glycosidic linkage normally present in lactose.The enzyme was completely inhibited by transition metal ions (Cu2+, Fe3+, Fe2+, Zn2+) and partially inhibited by high concentrations of glucose and galactoseas well as Ca2+. It was activated by most of the alkaline earth metal ions (NA+, K+, Li+). Oligosaccharides were formed at the different levels of lactose concentrations reaching more than 20% for high lactose concentration (20%). Three types of oligosaccharides were formed in significant concentrations detected by HPLC analysis. © 1991, American Dairy Science Association. All rights reserved.