Isolation and Characterization of Cinobufagin 3-Glutaroyl-L-arginine Ester from Bufo bufo gargarizans Cantor

Abstract

A new bufotoxin having glutaric acid as a dicarboxylic acid moiety was found in the Korean toad, Bufo bufo gargarizans cantor. this new bufotoxin was separated from the dried toad by usual chromatographic methods including reversed phase high-performance liquid chromatography, and its structure was elucidated to be cinobufagin 3-glutaroyl-L-arginine ester by degradative and synthetic means. In addition, seven known bufogenins and eight known bufotoxins were isolated and characterized. © 1985, The Pharmaceutical Society of Japan. All rights reserved.