Comparison of mechanical and electrical activity and interstitial cells of Cajal in urinary bladders from wild-type and W/W v mice

Abstract

Background and purpose: W/W v and wild-type murine bladders were studied to determine whether the W/W v phenotype, which causes a reduction in, but not abolition of, tyrosine kinase activity, is a useful tool to study the function of bladder interstitial cells of Cajal (ICC). Experimental approach: Immunohistochemistry, tension recordings and microelectrode recordings of membrane potential were performed on wild-type and mutant bladders. Key results: Wild-type and W/W v detrusors contained c-Kit- and vimentin-immunopositive cells in comparable quantities, distribution and morphology. Electrical field stimulation evoked tetrodotoxin-sensitive contractions in wild-type and W/W v detrusor strips. Atropine reduced wild-type responses by 50% whereas a 25% reduction occurred in W/W v strips. The atropine-insensitive component was blocked by pyridoxal-5-phosphate- 6-azophenyl-2′,4′-disulphonic acid in both tissue types. Wild-type and W/W v detrusors had similar resting membrane potentials of -48 mV. Spontaneous electrical activity in both tissue types comprised action potentials and unitary potentials. Action potentials were nifedipine-sensitive whereas unitary potentials were not. Excitatory junction potentials were evoked by single pulses in both tissues. These were reduced by atropine in wild-type tissues but not in W/W v preparations. The atropine-insensitive component was abolished by pyridoxal-5-phosphate-6-azophenyl-2′,4′- disulphonic acid in both preparations. Conclusions and implications: Bladders from W/W v mice contain c-Kit- and vimentin-immunopositive ICC. There are similarities in the electrical and contractile properties of W/W v and wild-type detrusors. However, significant differences were found in the pharmacology of the responses to neurogenic stimulation with an apparent up-regulation of the purinergic component. These findings indicate that the W/W v strain may not be the best model to study ICC function in the bladder. Mandarin translation of abstract. © 2009 The British Pharmacological Society.