RAPD-PCR primer selection to analyze genetic diversity of Cinnamon plan

Abstract

RAPD is one of the DNA markers that utilize the principle of Polymerase Chain Reaction (PCR) machine which can amplify specific DNA sequences in vitro. The primary selection is made to display the DNA band pattern which can be used to obtain polymorphism information. This study aims to determine the RAPD-PCR primer that can be used to differentiate accession of cinnamon bark in Jambi Province, Indonesia. RAPD-PCR primer selection was carried out by using 100 primers which produces a polymorphic DNA band. Ten primers were identified to produce polymorphic DNA bands for cinnamon bark DNA namely: OPE-6, OPE-20, OPH-7, OPH-14, OPH-19, OPM-2, OPM-5, OPM-6, OPM-16 and OPM-19.