Sitting vs. supine ultrasound measurements of the vastus medialis: correlations with MRI measurements and age considerations

Abstract

Background: Muscle thickness (MT) and echo intensity (EI) measurements are ultrasound alternatives to magnetic resonance imaging (MRI) for evaluating muscle quantity and quality. The vastus medialis (VM) is a clinically important muscle, and assessment methods that most accurately reflect its quantity and quality are required. This study aimed to examine the correlation between MT and EI measured in the supine and sitting postures with corresponding MRI-measured muscle quantity and quality indices. Methods: In total, 134 adults (91 older and 43 young) participated in this study. Ultrasound images of the VM were acquired in the supine and sitting postures, and MT and EI were measured. The cross-sectional area (CSA), muscle volume (MV), and intramuscular adipose tissue (intraMAT) of the VM were evaluated from MRI images using T1-weighted and Dixon methods. Pearson’s coefficients were used to quantify the correlation strength amongst pairs of dependent variables. Meng’s test was used to test for correlation coefficient differences between the two measurement postures (supine and sitting). Results: The correlation coefficients amongst MT, CSA, and MV were significantly higher in the sitting posture than in the supine posture. EI measured in the supine and sitting postures correlated significantly with intraMAT, and in young individuals, these correlation coefficients were significantly higher in the sitting posture than in the supine posture. Conclusions: These findings suggest that assessment of VM muscle quantity in the sitting posture is superior for young and older individuals, and assessment of VM muscle quality in the sitting posture is most effective in younger individuals.