Conditioning factor affecting growth in plant cells in culture

Abstract

Crude extracts of conditioning factor (CF) of plant cell cultures were obtained by dialysis filtration of Daucus carota L. plant cells. The lyophilized extract is stable in freezer. It is resistant to boiling, to acid or alkaline pH, it is strongly hydrophilic and it is species-unspecific. After Sephadex filtration CF shows a molecular weight of approx. 700 daltons. The effect of CF on the increase of plating efficiency can be mimicked by Brassinosteroid. However, these compounds act in a different way and show a synergic effect when tested together. © 1987.