Isolation and purification of oligopeptides from Ruditapes philippinarum and its inhibition on the growth of DU-145 cells in vitro

Abstract

Ruditapes philippinarum is a member of the Veneridae family of marine bivalve molluscs. RPOI-1 (Ruditapes philippinarum oligopeptide) is a tetrapeptide that can be extracted from Ruditapes philippinarum by means of enzymolysis. This study showed that RPOI-1 strongly inhibits proliferation and induces apoptosis in DU-145 human prostate cancer cells. When cells were treated with varying concentrations of RPOI-1, significant inhibition of proliferation was detected by an MTT assay, and sub-G1 and G2/M phase cell cycle arrest was observed using flow cytometric (FCM) analysis. Furthermore, morphological changes characteristic of apoptosis and an increase in the proportion of apoptotic cells were observed using double sequential acridine orange/ethidium bromide staining, FCM analysis and transmission election microscopy. FCM studies showed that exposing DU-145 cells to 10, 20 and 30 mg/ml RPOI-1 for 24 h increased the percentage of cells in the early-stages of apoptotis in a dose-dependent manner, with the numbers rising from 3.01% in the control group to 13.40% in the group treated with the highest dose.