New Dogashima Medium as Subculture Medium Improve the Growth of Vanda tricolor Shoots from Embryogenesis

Abstract

Vanda tricolor is an orchid endemic to Mount Merapi in Yogyakarta Indonesia. Efforts to propagate Vanda tricolor through in vitro culture have been carried out to restore the Vanda tricolor population that has decreased due to the eruption and human exploitation. The shoots that have been obtained must be transferred to a new medium by subculture. This study aimed to obtain the best medium for growth and enlargement of Vanda tricolor orchid shoot from organogenesis and embryogenesis by in vitro culture. This research is an experimental study conducted in a laboratory using a completely randomized design with a single factor, namely shoot growth from organogenesis and embryogenesis on various subculture medium. The explants used were shoot explants from organogenesis and shoot explants from embryogenesis which were subcultured into Murashige and Skoog Medium, Vacint and Went Medium and New Dogashima Medium without plant growth regulator. 0.2 g/l activated charcoal and 0.5 ml/l Plant Preservative Mixture was added into each medium. Each treatments was repeated 3 times, each replication consist of 3 samples. The results showed that the shoots from embryogenesis subcultured into the New Dogashima Medium without growth regulators was the best treatment for the enlargement of Vanda tricolor orchid.