ANTIDIABETIC ACTIVITY TEST FOR LEAVES EXTRACT OF CASSIA SIAMEA. LAMK

Abstract

Cassia siamea Lamk has been used as a traditional medicine in Indonesia including to treat diabetes mellitus (DM). This study aimed to investigate in vivo anti-diabetic activities of the ethanol extract, ethyl acetate fraction and n-hexane fraction of Cassia siamea Lamk in alloxan-induced mice. The study concluded that: (1) the ethanol extract and n-hexane fraction of Cassia siamea Lamk (Juar) leaves have anti-diabetic activity in Webster albino mice induced with alloxan, (2) The extract of Cassia siamea Lamk leaves, the fractions of 500 mg ethyl acetate and 500 mg n-Hexana of Cassia siamea Lamk provided better performances in lowering blood glucose levels compared to Ethanol extracts both 500 mg and 1000 mg. In the form of ethyl acetate and n-hexane fraction at a dose of 150 mg/kg BW provided the highest anti-diabetic activity compared to the other test groups that are able to decrease blood sugar level by 10.25% and 9.98% respectively. Its effect is equivalent to glibenclamide at a dose of 0.65 mg/kg BW which can lower blood sugar levels by 9.27%. Thus Cassia siamea Lamk leaf is very potential as an alternative drug antidiabetes mellitus, and (3) the 1000 mg Ethanol extract, 150 mg Ethyl acetate and 150 mg n-Hexana had no difference effects in lowering blood glucose levels compared to the anti-diabetic chemical drug glibenclamide.