Exploring drying pattern of a sessile droplet of genomic DNA in the presence of hematite nanoparticles

Abstract

For the first time, drying pattern of a sessile droplet of genomic DNA, in the presence of hematite nanoparticles was sighted by polarizing optical microscopy (POM) in this research article. POM results indicated that only at an appreciably high concentration of hematite nanoparticles dried pattern of deoxyribonucleic acid from calf thymus (CT-DNA) was altered. Iron hybridized cetylpyridinium chloride was utilized for the preparation of iron oxide nanoparticles through hydrothermal method. Fourier transforms infrared spectroscopy (FTIR) and powder x-ray diffraction (PXRD) studies confirmed the formation of highly crystalline hematite i.e. α-Fe2O3 nanoparticles. Morphology of the synthesized nanoparticle was visualized by transmission electron microscope (TEM) and field emission scanning electron microscope (FESEM), which revealed that nanoparticles were rhombohedral in shape with a size of 45 ± 10 nm. Based upon all the findings, hydrothermal growth mechanism was also proposed having bilayer protection of surfactant around the nanoparticles. UV-Vis spectroscopy and fluorescence spectroscopy were explored to study the affinity of thus prepared nanoparticles towards calf thymus deoxyribonucleic acid (CT-DNA). The low value of binding constant calculated from the spectroscopy data confirmed the weak interaction between nanoparticles and the CT-DNA.