Secondary metabolites production of epigallocatechin gallate through in vitro culture of camellia sinensis l with cinnamic acid precursors

Abstract

The secondary metabolite epigallocatechin gallate found in the Camellia sinensis L plant, which is bioactive, can be an antioxidant and can prevent cancer. The problem of epigallocatechin gallate presence from plants is dependent on land height, environmental temperature conditions, the need for intensive care, and relatively low production levels. Therefore, we need the technology of epigallocatechin gallate production through in vitro culture techniques. The study aimed to obtain a technique of secondary metabolites production of epigallocatechin gallate through culture in vitro by optimizing the medium and providing precursors. The method used is to initiate callus by adding growth regulators and cinnamic acid precursors. After that, the growth of callus was observed then tested both qualitatively and quantitatively. The result obtained is a compact form of callus containing epigallocatechin gallate. This research suggests that the use of cinnamic acid precursors in vitro culture techniques of Camellia sinensis L in a relatively short period can increase wet callus weight.