Isolation, Characterization and the Biological Activity of Some Natural Components of Marine Sea Cucumber and Orange Peel

Abstract

Natural components thought to have a particular benefit nowadays. They are playing a necessary role in health attention. Sea cucumber is theorized to be a significant source of bioactive components. Moreover, Flavonoid glycosides are a group of polyphenols with different glycoside substituent that possesses diverse pharmacological activities and can help management coronavirus. So, this study aimed to extract saponin and polysaccharides from sea cucumber, also, extract both of naringin, hesperidin from orange peel and convert hesperidin to hesperetin then chemical analysis of all extracted compounds was performed to confirm their structure and evaluate their antioxidant, antibacterial and antitumor activity. The concentrations of saponin, naringen, hesperidin, hesperetin, and ascorbic acid, which scavenged 50% of DPPH radicals, were 10.50, 0.13, 0.13, 0.66, and 0.0025 mg/ml respectively. Furthermore, Cell viability of saponin, hesperidin and hespertin showed a growth inhibitory effect, IC50 28.78, 236.40 and 73.99. The obtained data indicated that sea cucumber saponin, polysaccharides, and orange peels, may provide a promising new therapeutic approach to HEPG2 cancer cells. Also, these compounds were effective antioxidant, so they may be effective and scavenge free radicals which resulted from the disease. Keywords: