Rapid determination of chloramphenicol residues in aquaculture tissues by immunochromatographic assay

Abstract

An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g-1 for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R2 = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site. 2007 © The Japan Society for Analytical Chemistry.