Viability of Osteochilus Hasselti Padjadjaran Strain Against Aeromonas Hydrophila Infection

Abstract

The purpose of this research is to reveal the viability of the new strain : nilem carp padjadjaran strain that challenged by Aeromonas hydrophila. The treatments are common carp (Cyprinus carpio), nilem carp (Osteochilus hasselti) and nilem carp (O. hasselti) padjadjaran strain that intraperitoneally challenged by A. hydrophila with 108 cfu ml-1. Parameters observed are mortality hours, gross pathological symptoms and haematological profile for 24 hour. Nilem padjadjaran strain mortality reached 100% after 10th hour postchallenge, while common carp and nilem reached 100% mortality in 14th hour postchallenge. Gross pathological symptoms showed reddish lesions, abdominal dropsy and exopthalmia, with first sign of reddish lesions is appeared in nilem padjadjaran strain at 3rd hour postchallenge. Feed responses of all fish are lost in 7th hour postchallenge. Nilem padjadjaran strain has no shock responses in 9th hour postchallenge. Total erythrocyte counts of common carp, nilem and nilem padjadjaran strain before challenged are 2,15 x 106, 2,45 x 106 dan 2,14 x 106 cells mm3-1, after 10th  hour postchallenge becomes 1,2 x 106, 1,69 x 106 dan 1,21 x 106 cells mm3 -1. Total leukocyte count of common carp, nilem and nilem padjadjaran strain before challenged are 6,48 x 104, 7,36 x 104 and 6,56 x 104 cells mm3-1, and tend to increase  in 3rd and 7th hour postchallenge (8,8 x 104, 9,12 x 104 dan 105 cells mm3-1 and 1,26 x 105, 1,3 x 105, 1,27 x 105 cells mm3-1), but in 10th hour postchallenge the total leukocyte count becomes decreased again (1,24 x 105, 1,26 x 105, 1,18 x 105 cells mm3 -1). Thus, nilem carp padjadjaran strain has no better immune performance than its parents.