Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro

Abstract

The aim of the present work was to study the effect of oestrogen receptor inhibitors (ICI 164.384; ICI and cyclofenil; CF) on proliferation, oestradiol synthesis, aromatase expression and telomerase activity (TA) of pig granulosa cells (GC) derived from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. Cells were treated with anioestrogens for 48 h in basic (medium only) as well as FSH-stimulated conditions. Antioestrogens applied individually and in a combination significantly (P<0.01) decreased proliferative potential of LF-GC Antioestrogens applied in a combination increased (P<0.05-0.01) 17-β oestradiol synthesis in small and large follicle GC Antioestrogens applied individually and in a combination caused an increase (P<0.01) of aromatase gene expression in FSH-stimulated conditions as well as in basic conditions. Moreover, antioestrogens increased (P